Journal: Behavioural brain research
Article Title: Chronic methamphetamine exposure exerts few effects on the iTat mouse model of HIV, but blocks Tat expression-induced slowed reward retrieval
doi: 10.1016/j.bbr.2022.114109
Figure Lengend Snippet: Tat expression increases calbindin expression in the ventral tegmental area. Neither genotype nor drug exposure altered calbindin expression in the prefrontal cortex (A), hippocampus (B), or nucleus accumbens (C). In the ventral tegmental area, Tat expression increased calbindin expression, not otherwise influenced by prior methamphetamine (METH) exposure (D). Data presented as individual data points as well as mean ± S.E.M., * denotes p < 0.05 in Tat+ vs. Tat- mice after doxycycline treatment to both groups.
Article Snippet: 5 μm thick sagittal sections were processed for immunostaining using a rabbit polyclonal anti-Calbindin antibody (MilliporeSigma, Burlington, MA, USA) and the signal was detected with the ImmPRESS peroxidase-micropolymer anti rabbit secondary antibody and ImmPACT DAB substrate (Vector ® Laboratories, Burlingame, CA, USA).
Techniques: Expressing